Direct sample injection from a syringe needle into a separation capillary

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F18%3A43916921" target="_blank" >RIV/00216208:11120/18:43916921 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10382492

Result on the web

<a href="https://doi.org/10.1016/j.aca.2018.07.026" target="_blank" >https://doi.org/10.1016/j.aca.2018.07.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2018.07.026" target="_blank" >10.1016/j.aca.2018.07.026</a>

Result language

angličtina

Original language name

Direct sample injection from a syringe needle into a separation capillary

Original language description

An automatic micro-injector was developed for electrophoretic analysis of a microlitre amount of clinical samples, enabling injection of the sample from a Hamilton syringe. The outlet of the syringe needle is located directly opposite the inlet of the separation capillary at a defined distance of the order of hundreds of μm in the injection space. During the injection, the background electrolyte is forced out by air from this space and a drop of the sample is forced out of the syringe by a micro-pump so that it is caught at the entrance to the capillary. From the drop the sample is injected into the capillary by applying a negative pressure pulse or simply by spontaneous injection. The injection space is then filled with background electrolyte, which washes away excess sample and separation is commenced. The injector was tested in electrophoretic separation of a model sample with equimolar concentrations of 100 μM NH4 +, K+, Na+, Mg2+ and Li+ in a short capillary with total/effective length of 16.5/11.5 cm. The repeatability of the migration time and peak area expressed as the RSD value is 2% and 4%, respectively. The practical applicability of the injector was verified on the determination of the antiparasitic pentamidine in 10 μL of rat plasma. Electrophoretic separation of pentamidine was performed in 100 mM of acetic acid/NaOH at pH 4.55, the sample consumption per analysis is 125 nL, the separation time is 45 s and the attained LOQ using contactless conductivity detection is 8 μM.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA15-03139S" target="_blank" >GA15-03139S: New Electrophoretic Approaches in Studies of Obesity and Diabetes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

—

Volume of the periodical

1042

Issue of the periodical within the volume

December

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

133-140

UT code for WoS article

000448290800016

EID of the result in the Scopus database

2-s2.0-85049915456