Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic beta-cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43918474" target="_blank" >RIV/00216208:11120/19:43918474 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3390/ijms20143441" target="_blank" >https://doi.org/10.3390/ijms20143441</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms20143441" target="_blank" >10.3390/ijms20143441</a>
Alternative languages
Result language
angličtina
Original language name
Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic beta-cells
Original language description
Saturated fatty acids (FAs) induce apoptosis in the human pancreatic NES2Y β-cell line while unsaturated FAs have nearly no detrimental effect. Moreover, unsaturated FAs are capable of inhibiting the pro-apoptotic effect of saturated FAs. Hypoxia is also known to have deleterious effects on β-cells function and viability. In the present study, we have tested the modulatory effect of hypoxia on the effect of FAs on the growth and viability of the human pancreatic NES2Y β-cells. This study represents the first study testing hypoxia effect on effects of FAs in pancreatic β-cells as well as in other cell types. We showed that hypoxia increased the pro-apoptotic effect of saturated stearic acid (SA). Endoplasmic reticulum stress signaling seemed to be involved while redistribution of FA transporters fatty acid translocase/cluster of differentiation 36 (FAT/CD36) and fatty acid-binding protein (FABP) do not seem to be involved in this effect. Hypoxia also strongly decreased the protective effect of unsaturated oleic acid (OA) against the pro-apoptotic effect of SA. Thus, in the presence of hypoxia, OA was unable to save SA-treated β-cells from apoptosis induction. Hypoxia itself had only a weak detrimental effect on NES2Y cells. Our data suggest that hypoxia could represent an important factor in pancreatic β-cell death induced and regulated by FAs and thus in the development of type 2 diabetes mellitus.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA18-10144S" target="_blank" >GA18-10144S: Hypoxia-induced Mitochondrial Adaptations as the Unifying Factor of Type 2 Diabetes Development in Sleep Apnea Syndrome</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
14
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
"Article 3441"
UT code for WoS article
000480449300065
EID of the result in the Scopus database
2-s2.0-85070474802