Insight into the distribution of amino groups along the chain of chemically deacetylated hyaluronan
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43918628" target="_blank" >RIV/00216208:11120/19:43918628 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/19:10396483
Result on the web
<a href="https://doi.org/10.1016/j.carbpol.2019.115156" target="_blank" >https://doi.org/10.1016/j.carbpol.2019.115156</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carbpol.2019.115156" target="_blank" >10.1016/j.carbpol.2019.115156</a>
Alternative languages
Result language
angličtina
Original language name
Insight into the distribution of amino groups along the chain of chemically deacetylated hyaluronan
Original language description
Deacetylated hyaluronan (daHA) containing reactive free amino groups is an important intermediate for further modification. Comparing direct and indirect NMR and HPLC to characterize the degree of HA deacetylation (DD), direct NMR approach using area ratio of anomeric CH and CH-NH2 groups was the most precise one. To describe the substitution pattern, daHA was selectively cleaved by nitrous acid generated in situ or hyaluronan lyase from Streptococcus pneumoniae. The resulting oligomers were identified by LC-ESI-MS. The experimental distribution of these oligomers was compared with theoretically expected random oligomer distribution. Independently on the starting HA molecular weight and deacetylation conditions, the experimental data differed from the random distribution model and suggested that deacetylation of certain N-acetyl-D-glucosamine had reduced the probability of deacetylation at the neighbouring disaccharide. This phenomenon was explained by conformational changes of HA caused by intra- and intermolecular interactions between positively charged amino and negatively charged carboxylic groups.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Carbohydrate Polymers
ISSN
0144-8617
e-ISSN
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Volume of the periodical
225
Issue of the periodical within the volume
December
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
"Article 115156"
UT code for WoS article
000485109100026
EID of the result in the Scopus database
2-s2.0-85071400051