ALCAT1 Overexpression Affects Supercomplex Formation and Increases ROS in Respiring Mitochondria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43919452" target="_blank" >RIV/00216208:11120/19:43919452 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1155/2019/9186469" target="_blank" >https://doi.org/10.1155/2019/9186469</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2019/9186469" target="_blank" >10.1155/2019/9186469</a>
Alternative languages
Result language
angličtina
Original language name
ALCAT1 Overexpression Affects Supercomplex Formation and Increases ROS in Respiring Mitochondria
Original language description
Cardiolipin (CL) is a multifunctional dimeric phospholipid that physically interacts with electron transport chain complexes I, III, and IV, and ATP synthase (complex V). The enzyme ALCAT1 catalyzes the conversion of cardiolipin by incorporating polyunsaturated fatty acids into cardiolipin. The resulting CL species are said to be more susceptible to oxidative damage. This is thought to negatively affect the interaction of cardiolipin and electron transport chain complexes, leading to increased ROS production and mitochondrial dysfunction. Furthermore, it is discussed that ALCAT1 itself is upregulated due to oxidative stress. Here, we investigated the effects of overexpression of ALCAT1 under different metabolic conditions. ALCAT1 is located at the ER and mitochondria, probably at contact sites. We found that respiration stimulated by galactose supply promoted supercomplex assembly but also led to increased mitochondrial ROS levels. Endogeneous ALCAT1 protein expression levels showed a fairly high variability. Artificially induced ALCAT1 overexpression reduced supercomplex formation, further promoted ROS production, and prevented upregulation of coupled respiration. Taken together, our data suggest that the amount of the CL conversion enzyme ALCAT1 is critical for coupling mitochondrial respiration and metabolic plasticity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oxidative Medicine and Cellular Longevity
ISSN
1942-0900
e-ISSN
—
Volume of the periodical
2019
Issue of the periodical within the volume
December
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
"Article 9186469"
UT code for WoS article
000503780800006
EID of the result in the Scopus database
2-s2.0-85076926533