Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43920277" target="_blank" >RIV/00216208:11120/20:43920277 - isvavai.cz</a>

Alternative codes found

RIV/00023698:_____/20:N0000027

Result on the web

<a href="https://doi.org/10.1088/1758-5090/ab8f36" target="_blank" >https://doi.org/10.1088/1758-5090/ab8f36</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1758-5090/ab8f36" target="_blank" >10.1088/1758-5090/ab8f36</a>

Result language

angličtina

Original language name

Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle

Original language description

A common shortcoming of current tissue engineered constructs is the lack of a functional vasculature, limiting their size and functionality. Prevascularization is a possible strategy to introduce vascular networks in these constructs. It includes among others co-culturing target cells with endothelial (precursor) cells that are able to form endothelial networks through vasculogenesis. In this paper, we compared two different prevascularization approaches of bio-artificial skeletal muscle tissue (BAM) in vitroandin vivo. In a one-stage approach, human muscle cells were directly co-cultured with endothelial cells in 3D. In a two-stage approach, a one week old BAM containing differentiated myotubes was coated with a fibrin hydrogel containing endothelial cells. The obtained endothelial networks were longer and better interconnected with the two-stage approach. We evaluated whether prevascularization had a beneficial effect onin vivoperfusion of the BAM and improved myotube survival by implantation on the fascia of thelatissimus dorsimuscle of NOD/SCID mice for 5 or 14 d. Alsoin vivo, the two-stage approach displayed the highest vascular density. At day 14, anastomosis of implanted endothelial networks with the host vasculature was apparent. BAMs without endothelial networks contained longer and thicker myotubesin vitro, but their morphology degradedin vivo. In contrast, maintenance of myotube morphology was well supported in the two-stage prevascularized BAMs. To conclude, a two-stage prevascularization approach for muscle engineering improved the vascular density in the construct and supported myotube maintenancein vivo.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

—

Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biofabrication

ISSN

1758-5082

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

"Article 035021"

UT code for WoS article

000542188000001

EID of the result in the Scopus database

2-s2.0-85086522795