Genistein does not Inhibit TGF-beta 1-Induced Conversion of Human Dermal Fibroblasts to Myofibroblasts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F21%3A43922691" target="_blank" >RIV/00216208:11120/21:43922691 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/21:10434932

Result on the web

<a href="https://doi.org/10.33549/physiolres.934666" target="_blank" >https://doi.org/10.33549/physiolres.934666</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934666" target="_blank" >10.33549/physiolres.934666</a>

Result language

angličtina

Original language name

Genistein does not Inhibit TGF-beta 1-Induced Conversion of Human Dermal Fibroblasts to Myofibroblasts

Original language description

Transforming growth factor beta 1 (TGF-beta 1) is a pro-fibrotic cytokine with a key role in wound repair and regeneration, including induction of fibroblast-to-myofibroblast transition. Genistein is a naturally occurring selective estrogen receptor modulator with promising anti-fibrotic properties. In the present study we aimed to investigate whether genistein modulates TGF-beta 1 (canonical and non-canonical) signaling in normal dermal fibroblasts at the protein level (Western blot and immunofluorescence). We demonstrated that TGF-beta 1 induces the myofibroblast-like phenotype in the studied fibroblast signaling via canonical (SMAD) and non-canonical (AKT, ERK1/2, ROCK) pathways. Genistein induced only ERK1/2 expression, whereas the combination of TGF-beta 1 and genistein attenuated the ERK1/2 and ROCK signaling. Of note, the other studied pathways remained almost unaffected. From this point of view, genistein does not impair conversion of normal fibroblasts to myofibroblast-like cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30105 - Physiology (including cytology)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

—

Volume of the periodical

70

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

815-820

UT code for WoS article

000729810300015

EID of the result in the Scopus database

2-s2.0-85122331827