Caffeic acid does not protect human pancreatic β cells against stearic acid-induced ER stress and apoptosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F24%3A43926915" target="_blank" >RIV/00216208:11120/24:43926915 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/ejlt.202300206" target="_blank" >https://doi.org/10.1002/ejlt.202300206</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ejlt.202300206" target="_blank" >10.1002/ejlt.202300206</a>
Alternative languages
Result language
angličtina
Original language name
Caffeic acid does not protect human pancreatic β cells against stearic acid-induced ER stress and apoptosis
Original language description
Long-term elevation of saturated fatty acids in blood has a deleterious effect on pancreatic beta-cell function and survival, leading to endoplasmic reticulum (ER) stress and apoptosis. This fundamentally contributes to type 2 diabetes development. Caffeic acid (CA) was found to protect various cell types against several proapoptotic stimuli, including fatty acids. However, its potential protective effect against fatty acid-induced apoptosis was not ascertained in pancreatic beta cells yet. Therefore, the objective of this study was to examine this in the human pancreatic beta-cell lines NES2Y and 1.1B4. In both cell lines, CA did not modify the effect of saturated stearic acid (SA) on beta-cell growth and viability. At higher concentrations, CA significantly even intensified the adverse effect of SA. Consistent with this, CA did not exhibit any inhibitory effect on SA-induced markers of ongoing apoptosis as well as ER stress. At higher concentrations, CA again slightly potentiated the effect of SA. CA applied alone was well tolerated up to 1 mM; however, at higher concentrations, it had detrimental effects in both cell lines. To conclude, we have shown that the treatment with caffeic acid has no inhibitory effect on SA-induced ER stress and apoptosis in the human pancreatic beta cells. Moreover, at higher concentrations, CA has proapoptotic potential.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/LX22NPO5104" target="_blank" >LX22NPO5104: National Institute for Research of Metabolic and Cardiovascular Diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Lipid Science and Technology
ISSN
1438-7697
e-ISSN
1438-9312
Volume of the periodical
126
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
2300206
UT code for WoS article
001197065700001
EID of the result in the Scopus database
2-s2.0-85190394651