Assessment of lymphocyte proliferation: CFSE kills dividing cells and modulates expression of activation markers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F09%3A5395" target="_blank" >RIV/00216208:11130/09:5395 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/09:5395
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Assessment of lymphocyte proliferation: CFSE kills dividing cells and modulates expression of activation markers
Original language description
The measurement of cell proliferation after mitogenic stimulation is an important parameter used in diagnosis of immunodeficiencies in clinical laboratory as well as in various fields of lymphocyte research. Recent methods try to overcome the radioactiveassay using tritiated thymidine (H-3) by flow-cytometric methods using different fluorochromes such as CFSE or even to substitute these direct methods by tracing the expression of cell-membrane activation markers associated with various steps of proliferation cycle. In our study we compared the H-3 assay with CFSE-staining method and expression of activation markers (CD69, HLA-DR, CD25, CD27, CD71, CD152, CD134 and CD195) on a sample of 128 consecutive patients and healthy controls evaluated in clinical laboratory.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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