Identification of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in patients with erythema migrans

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F09%3A5562" target="_blank" >RIV/00216208:11130/09:5562 - isvavai.cz</a>

Alternative codes found

RIV/75010330:_____/09:00008345

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Identification of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in patients with erythema migrans

Original language description

Anaplasma phagocytophilum has been first isolated from the blood of two Czech patients simultaneously with a cultivation of Borrelia burgdorferi sensu lato from their erythema migrans lesions. Cultivation of different Borrelia spp. from 12 erythema migrans biopsies, from 2 blood, one liquor and one placenta sample in BSK-H medium was successful. Adapted conventional methods targeting 16S rRNA and OspA genes for real-time polymerase chain reaction (PCR) and partial sequencing of these genes together withmicroscopical examinations of the blood smears provided a direct detection of the B. afzelii, B. burgdorferi, B. garinii, B. valaisiana and B. bissettii in the skin, B. garinii in the blood, placenta and liquor in 24 (36.3 %) patients, and A. phagocytophilum in 10 (15 %) patients with erythema migrans. Positive indirect IgM immunofluorescence against Anaplasma sp. was obtained in 7 cases, specific IgG antibodies were detected in 12 patients.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FO - Dermatology and venereology

OECD FORD branch

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Project

<a href="/en/project/NR8263" target="_blank" >NR8263: Participation of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in skin manifestations</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

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UT code for WoS article

000268573600012

EID of the result in the Scopus database

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