Generation of hydrogen peroxide in the developing rat heart: the role of elastin metabolism

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F11%3A7167" target="_blank" >RIV/00216208:11130/11:7167 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/11:00369506
Result on the web
<a href="http://www.ncbi.nlm.nih.gov/pubmed/21769453" target="_blank" >http://www.ncbi.nlm.nih.gov/pubmed/21769453</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Generation of hydrogen peroxide in the developing rat heart: the role of elastin metabolism
Original language description
Reports describing production of reactive oxygen species in neonatal heart are missing. As lysyl oxidase is potentially important source of H(2)O(2), we studied its role during ontogenic development of rat heart. H(2)O(2) was detected in thin sections ofdeveloping rat heart by fluorescence microscopy with the use of fluorescence probe 2'-7'-dichlorofluorescin. The experimental design comprised foetuses 21 days after conception, and then the animals sampled on the 1st, 4th, 7th, 10th, 15th, 30th and 60th day after birth. We also used 7-month-old animals as an example of ageing effects. Since the day 4 on, H(2)O(2) was produced only extracellularly up to the day 15, between days 30 and 60 intracellular production was detected as well, and in 7-month-oldanimals only extracellular production was observed. The specific inhibitors of lysyl oxidase almost completely quenched the H(2)O(2)-dependent fluorescence. Starting from day 7, blue autofluorescence specific to oxidized proteins develop
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
<a href="/en/project/1M0510" target="_blank" >1M0510: Centre for cardiovascular research</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)