Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10361006" target="_blank" >RIV/00216208:11130/17:10361006 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/17:00097555 RIV/00159816:_____/17:00067196 RIV/00064203:_____/17:10361006
Result on the web
<a href="http://dx.doi.org/10.1089/mdr.2016.0159" target="_blank" >http://dx.doi.org/10.1089/mdr.2016.0159</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/mdr.2016.0159" target="_blank" >10.1089/mdr.2016.0159</a>
Alternative languages
Result language
angličtina
Original language name
Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis
Original language description
Aim: To perform a retrospective analysis of the high occurrence of Clostridium difficile infection in the surgical department of a Czech tertiary care hospital and to identify weaknesses in C. difficile infection (CDI) prevention and control policies. Methods: Clinical and epidemiological data on eleven CDI cases were collected. C. difficile isolates were characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat analysis (MLVA), gyrA gene fragment sequencing, and erm(B) fragment PCR amplification. Antibiotic susceptibility to metronidazole, vancomycin, ciprofloxacin, moxifloxacin, and clindamycin was tested. Findings: Eleven CDI cases were caused by C. difficile PCR ribotype 001 strains. These strains revealed two different MLVA profiles with 11 tandem repeat differences. All isolates were susceptible to metronidazole and vancomycin and resistant to ciprofloxacin (MIC 32mg/L), moxifloxacin (MIC 32mg/L), and clindamycin (MIC 256mg/L). All isolates revealed amino acid substitution Thr82Ile, in the GyrA and were erm(B) negative. Conclusion: Two fluoroquinolone and clindamycin-resistant C. difficile PCR ribotype 001 strain clusters occurred at one of the surgical departments of a tertiary care hospital. Ineffective decontamination with suboptimal concentration and time of exposure of sporicidal disinfectants may have resulted in C. difficile transmission.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbial Drug Resistance
ISSN
1076-6294
e-ISSN
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Volume of the periodical
23
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
609-615
UT code for WoS article
000404988200010
EID of the result in the Scopus database
2-s2.0-85022175936