Analysis of circulating miRNAs in patients with familial hypercholesterolaemia treated by LDL/Lp(a) apheresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10365939" target="_blank" >RIV/00216208:11130/17:10365939 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/17:10365939 RIV/00179906:_____/17:10365939 RIV/00064203:_____/17:10365939 RIV/00023001:_____/17:00076209

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S156756881730079X?via%3Dihub" target="_blank" >http://www.sciencedirect.com/science/article/pii/S156756881730079X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.atherosclerosissup.2017.05.037" target="_blank" >10.1016/j.atherosclerosissup.2017.05.037</a>

Result language

angličtina

Original language name

Analysis of circulating miRNAs in patients with familial hypercholesterolaemia treated by LDL/Lp(a) apheresis

Original language description

Background: LDL/Lp(a) apheresis therapy is a well-established method of aggressively lowering LDL and Lp(a). Recently, miRNAs have been discussed as markers of vascular status including atherosclerosis. MiRNAs inhibit post-transcriptional processes through RNA duplex formation resulting in gene silencing or regulation of gene expression. Materials and methods: We measured a profile of 175 plasma-circulating miRNAs using pre-defined Serum/Plasma Focus Human microRNA PCR Panels in pooled samples of 11 subjects with familial hypercholesterolaemia under long-term apheresis treatment. Subsequently we analysed expressions of ten pre-selected miRNAs potentially involved in lipid homeostasis in the same group of subjects. We compared plasma-circulating miRNA levels isolated from peripheral blood collected immediately before and after apheresis. Results: The greatest differences in plasma levels were found in miR-451a, miR-16, miR-19a/b, miR-223 and miR-185. In subsequent individual miRNA assay we detected a significant increase in miR-33b levels after apheresis (P &lt; 0.05). Additionally, correlations between plasma lipids and miR-33a (P &lt; 0.04) and miR-122 (P &lt; 0.01) have been determined. Moreover, miR-122 levels in LDLR homozygotes were higher compared to heterozygotes after, but not before, apheresis treatment (P &lt; 0.04). Conclusions: LDL/Lp(a) apheresis has an impact on miRNAs associated with lipid homeostasis and vascular status.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Atherosclerosis, Supplements

ISSN

1567-5688

e-ISSN

—

Volume of the periodical

30

Issue of the periodical within the volume

November

Country of publishing house

IE - IRELAND

Number of pages

7

Pages from-to

128-134

UT code for WoS article

000415625000019

EID of the result in the Scopus database

2-s2.0-85032457877