Severe, but not mild heat-shock treatment induces immunogenic cell death in cancer cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10373886" target="_blank" >RIV/00216208:11130/17:10373886 - isvavai.cz</a>

Alternative codes found

RIV/00064203:_____/17:10373886

Result on the web

<a href="https://doi.org/10.1080/2162402X.2017.1311433" target="_blank" >https://doi.org/10.1080/2162402X.2017.1311433</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/2162402X.2017.1311433" target="_blank" >10.1080/2162402X.2017.1311433</a>

Result language

angličtina

Original language name

Severe, but not mild heat-shock treatment induces immunogenic cell death in cancer cells

Original language description

The mechanisms of immunogenicity underlying mild heat-shock (mHS) treatment &lt; 42 degrees C of tumor cells are largely attributed to the action of heat-shock proteins; however, little is known about the immunogenicity of tumor cells undergoing severe cytotoxic heat-shock treatment (sHS &gt; 43 degrees C). Here, we found that sHS, but not mHS (42 degrees C), induces immunogenic cell death in human cancer cell lines as defined by the induction of ER stress response and ROS generation, cell surface exposure of calreticulin, HSP70 and HSP90, decrease of cell surface CD47, release of ATP and HMGB1. Only sHS-treated tumor cells were efficiently killed and phagocytosed by dendritic cells (DCs), which was partially dependent on cell surface calreticulin. DCs loaded with mHS or sHS-treated tumor cells displayed similar level of maturation and stimulated IFNg-producing CD8(+)C T cells without any additional adjuvants in vitro. However, only DCs loaded with sHS-treated tumor cells stimulated antigen-specific CD4(+) T cells and induced higher CD8(+) T-cell activation and proliferation. sHS-treated murine cells also exposed calreticulin, HSP70 and HSP90 and activated higher DC maturation than mHS treated cells. Vaccination with sHS-treated tumor cells elicited protective immunity in mice. In this study, we defined specific conditions for the sHS treatment of human lung and ovarian tumor cells to arrive at optimal ratio between effective cell death, immunogenicity and content of tumor antigens for immunotherapeutic vaccine generation.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

OncoImmunology [online]

ISSN

2162-402X

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

—

UT code for WoS article

000402926200011

EID of the result in the Scopus database

2-s2.0-85019034133