The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10375851" target="_blank" >RIV/00216208:11130/18:10375851 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/18:10375851
Result on the web
<a href="https://doi.org/10.1016/j.jmii.2017.02.002" target="_blank" >https://doi.org/10.1016/j.jmii.2017.02.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmii.2017.02.002" target="_blank" >10.1016/j.jmii.2017.02.002</a>
Alternative languages
Result language
angličtina
Original language name
The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027
Original language description
Purpose: To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods. Methods: Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments. Susceptibility to moxifloxacin was determined by E-test. Results: Of 28 RT027-like isolates, seven RTs (016, 034, 075, 080, 153, 176 and 328), three WEBRIBO types (411, 475, AI-78) and three new profiles (F1-F3) were identified. MLVA revealed six clonal complexes (RTs 016, 027, 176 and F3). MLST showed eleven sequence types (1, 41, 47, 67, 95, 191,192, 223, 229, 264 and new ST). Twenty-two isolates (RTs 016, 080, 176, 328, F1, F2, F3 and WRTAI-78) carried Δ117 in the tcdC gene. Isolates of RTs 016, 027 and 176 were moxifloxacin resistant and harboured Thr82Ile in the GyrA. Conclusion: Our results show a high diversity within 28 Finnish RT027-like C. difficile isolates, with twelve CE-ribotyping profiles and eleven STs. MLVA revealed the regional spread of RTs 016, 027, 176 and F3. The presence of Δ117 in the tcdC gene in eight non-027 RTs highlights the importance of careful interpretation of the results from molecular systems targeting this site in the genome of C. difficile and the need of strain typing for epidemiological purposes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiology, Immunology and Infection
ISSN
1684-1182
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
3
Country of publishing house
TW - TAIWAN (PROVINCE OF CHINA)
Number of pages
8
Pages from-to
344-351
UT code for WoS article
000434423800007
EID of the result in the Scopus database
2-s2.0-85020082674