Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10379264" target="_blank" >RIV/00216208:11130/18:10379264 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/18:00496207 RIV/00216208:11140/18:10379264
Result on the web
<a href="https://doi.org/10.1038/s41598-018-31361-1" target="_blank" >https://doi.org/10.1038/s41598-018-31361-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-31361-1" target="_blank" >10.1038/s41598-018-31361-1</a>

Result language
angličtina
Original language name
Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation
Original language description
The neurohormones arginine-vasopressin (AVP) and oxytocin (OT) synthesised in supraoptic and paraventricular nuclei of neurohypophysis regulate lactation, systemic water homeostasis and nociception. Using transgenic rats expressing AVP and OT tagged with fluorescent proteins we demonstrate that both neurohormones are expressed in sensory neurones both in vitro, in primary cultures, and in situ, in the intact ganglia; this expression was further confirmed with immunocytochemistry. Both neurohormones were expressed in nociceptive neurones immunopositive to transient receptor potential vannilloid 1 (TRPV1) channel antibodies. The AVP and OT-expressing DRG neurones responded to AVP, OT, 50 mM K+ and capsaicin with [Ca2+](i) transients; responses to AVP and OT were specifically blocked by the antagonists of V-1 AVP and OT receptors. Probing the extracellular incubation saline with ELISA revealed AVP and OT secretion from isolated DRGs; this secretion was inhibited by tetanus toxin (TeNT) indicating the role for vesicular release. Expression of OT, but not AVP in DRG neurones significantly increased during lactation. Together, the results indicate novel physiological roles (possibly related to nociception and mood regulation) of AVP and OT in the sensory neurones.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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