Virome Sequencing of Stool Samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10384954" target="_blank" >RIV/00216208:11130/18:10384954 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/18:10384954
Result on the web
<a href="https://doi.org/10.1007/978-1-4939-8682-8_6" target="_blank" >https://doi.org/10.1007/978-1-4939-8682-8_6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-8682-8_6" target="_blank" >10.1007/978-1-4939-8682-8_6</a>
Alternative languages
Result language
angličtina
Original language name
Virome Sequencing of Stool Samples
Original language description
Next-generation sequencing has opened avenues to studying complex populations such as the bacteriome (all bacteria), mycobiome (all fungi), and virome (all viruses in a given sample). Viromes are less often investigated as compared to bacteriomes. The reasons are mostly methodological: because no common pan-viral sequence signature exists, metagenomic sequencing remains the only option. This brings about the need of laborious virus enrichment, multiple signal amplification steps with virtually no possibility of interim quality control, and complicated bioinformatic analysis of the ensuing sequence data. Nevertheless, over the past decade virome sequencing has been enormously successful in identifying new agents in human and animal diseases, and in characterizing viruses in various ecological niches. Recently, virome sequencing has been also employed in studies of non-infectious diseases, which has brought about new challenges of sensitivity, costs, and reproducibility in testing of large sets of samples. Here, we present a detailed protocol that has been utilized in virome studies where hundreds of samples had to be reliably tested in order to assess the association of the stool virome with susceptibility to type 1 diabetes, a non-infectious autoimmune disease.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
The Human Virome
ISBN
978-1-4939-8681-1
Number of pages of the result
25
Pages from-to
59-83
Number of pages of the book
274
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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