A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F21%3A10428125" target="_blank" >RIV/00216208:11130/21:10428125 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/21:10428125
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=54MXDroICT" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=54MXDroICT</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-1266-8_28" target="_blank" >10.1007/978-1-0716-1266-8_28</a>
Alternative languages
Result language
angličtina
Original language name
A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry
Original language description
Nitrotyrosine formation is caused by presence of reactive oxygen and nitrogen species. Nitration is a very selective process leading to specific modification of only a few tyrosines in protein molecule. 2D electrophoresis and western blotting techniques coupled with mass spectrometry are common methods used in analysis of proteome. Here we describe protocol for analysis of peroxynitrite-induced protein nitration in isolated mitochondria. Mitochondrial proteins are separated by 2D electrophoresis and transferred to nitrocellulose membrane. Membranes are then incubated with antibodies against nitrotyrosine. Positive spots are compared with corresponding Coomassie-stained gels, and protein nitration is confirmed with mass spectrometry techniques.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
30100 - Basic medicine
Result continuities
Project
<a href="/en/project/GAP303%2F11%2F0298" target="_blank" >GAP303/11/0298: Biochemical and morphological aspects of early postnatal development of rat heart and brain. The role of free radicals.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Molecular Biology
ISSN
1064-3745
e-ISSN
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Volume of the periodical
2276
Issue of the periodical within the volume
June
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
383-396
UT code for WoS article
—
EID of the result in the Scopus database
2-s2.0-85107188199