Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F12%3A10118804" target="_blank" >RIV/00216208:11140/12:10118804 - isvavai.cz</a>
Alternative codes found
RIV/49777513:23520/12:43914832
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jemt.21071/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jemt.21071/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jemt.21071" target="_blank" >10.1002/jemt.21071</a>

Result language
angličtina
Original language name
Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment
Original language description
Quantification of immunohistochemical results constitutes an important tool in the analysis of cells and tissue that is not readily replaced by other techniques. For reliable quantification, it is essential to consider factors such as tissue fixation andtissue sampling. We report a study on the model of the intestine of Isospora suis-infected piglets, in which we addressed (1) whether the quantity of detectable T cells in the intestinal mucosa is the same in formalin-, HOPE(R)-, and cryo-conserved material or whether the amounts of T cells at least correlate with one another; and (2) whether single jejunal segments differ in regard to the quantity of mucosal T cells and variability of lymphocyte infiltration. Quantification of T cells in histologicalsections of different parts of the jejunum of 15-22 day old piglets infected with I. suis was performed using an anti-CD3-antibody and stereological point counting. Area fractions of T-cell profiles per intestinal mucosa profile were high
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Project
<a href="/en/project/ME09090" target="_blank" >ME09090: Designing quantitative studies in histology - strategies of sampling and detection, testing of reliability and reproducibility</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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