Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 Carbapenemases by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F12%3A10121192" target="_blank" >RIV/00216208:11140/12:10121192 - isvavai.cz</a>
Result on the web
<a href="http://jcm.asm.org/content/50/7/2441.long" target="_blank" >http://jcm.asm.org/content/50/7/2441.long</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JCM.01002-12" target="_blank" >10.1128/JCM.01002-12</a>
Alternative languages
Result language
angličtina
Original language name
Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 Carbapenemases by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Original language description
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection of antimicrobial resistance, especially that conferred by -lactamases. Here we describe a modification of a previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members of the Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria producing no carbapenemase. The detection of carbapenemases by MALDI-TOF MS seems to be a powerful, quick, and cost-effective method for microbiological laboratories.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/NT11032" target="_blank" >NT11032: Analysis of Mobile Genetic Elements Carried Out Metallo-Beta-Lactamase Genes in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Microbiology
ISSN
0095-1137
e-ISSN
—
Volume of the periodical
50
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
3
Pages from-to
2441-2443
UT code for WoS article
000307360800042
EID of the result in the Scopus database
—