Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F15%3A10293982" target="_blank" >RIV/00216208:11140/15:10293982 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1128/JCM.03094-14" target="_blank" >http://dx.doi.org/10.1128/JCM.03094-14</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JCM.03094-14" target="_blank" >10.1128/JCM.03094-14</a>
Alternative languages
Result language
angličtina
Original language name
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Meropenem Hydrolysis Assay with NH4HCO3, a Reliable Tool for Direct Detection of Carbapenemase Activity
Original language description
A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT11032" target="_blank" >NT11032: Analysis of Mobile Genetic Elements Carried Out Metallo-Beta-Lactamase Genes in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Microbiology
ISSN
0095-1137
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
1731-1735
UT code for WoS article
000353036500041
EID of the result in the Scopus database
2-s2.0-84928017396