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Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-beta-Lactamase

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F17%3A10365177" target="_blank" >RIV/00216208:11140/17:10365177 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11150/17:10365177 RIV/00179906:_____/17:10365177 RIV/75010330:_____/17:00011866

  • Result on the web

    <a href="http://dx.doi.org/10.1128/AAC.01811-17" target="_blank" >http://dx.doi.org/10.1128/AAC.01811-17</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1128/AAC.01811-17" target="_blank" >10.1128/AAC.01811-17</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-beta-Lactamase

  • Original language description

    The objective of this study was to perform molecular surveillance for assessing the spread of carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six carbapenemase-producing isolates were recovered from 22 hospitals located throughout the country. Sequence type 357 (ST357) dominated (n = 120) among carbapenemase producers. One hundred seventeen isolates produced IMP-type (IMP-7 [n 116] and IMP-1 [n 1]) metallo--lactamases (MLs), 15 produced the VIM-2 ML, and the remaining isolates expressed the GES-5 enzyme. The blaIMP-like genes were located in three main integron types, with Inp110-like being the most prevalent (n 115). The two other IMP-encoding integrons (In1392 and In1393) have not been described previously. blaVIM-2-carrying integrons included In59-like, In56, and a novel element (In1391). blaGES-5 was carriedby In717. Sequencing data showed that In-p110-like was associated with a Tn4380-like transposon inserted in genomic island LESGI-3 in the P. aeruginosa chromosome. The other integrons were also integrated into the P. aeruginosa chromosome. These findings indicated the clonal spread of ST357 P. aeruginosa, carrying the IMP-7- encoding integron In-p110, in Czech hospitals. Additionally, the sporadic emergence of P. aeruginosa producing different carbapenemase types, associated with divergent or novel integrons, punctuated the ongoing evolution of these bacteria.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Antimicrobial Agents and Chemotherapy

  • ISSN

    0066-4804

  • e-ISSN

  • Volume of the periodical

    61

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

  • UT code for WoS article

    000416578900069

  • EID of the result in the Scopus database

    2-s2.0-85035057449