Evaluation of adhesion properties of lactobacilli probiotic candidates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F18%3A10375697" target="_blank" >RIV/00216208:11140/18:10375697 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s00706-017-2135-1" target="_blank" >https://doi.org/10.1007/s00706-017-2135-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-017-2135-1" target="_blank" >10.1007/s00706-017-2135-1</a>

Result language

angličtina

Original language name

Evaluation of adhesion properties of lactobacilli probiotic candidates

Original language description

Bacterial adhesion is a complex phenomenon implicated in the host-bacterial interaction that is pivotal for probiotic activity. Eight probiotic lactobacilli candidates (Lactobacillus reuteri, L. plantarum, L. mucosae, L. murinus) were screened for their ability to adhere to abiotic and biotic surfaces in vitro. Adhesion to hydrocarbons was used for hydrophobicity assessment. Three strains of L. reuteri and L. murinus C were evaluated as hydrophobic, others as intermediate. All tested strains were able to form the biofilm on polystyrene. L. mucosae D and L. reuteri E were tested for adhesion to epithelial cell lines (HeLa and Caco-2). Both were more adherent to HeLa than to Caco-2. The adhesivity degree in HeLa reached the highest value after 8 h of co-cultivation in both lactobacilli tested, then decreased. In Caco-2, adhesion was increased within 24 h from the beginning of the co-cultivation. Mucus-binding protein gene, implicated in adhesion, was detected in L. mucosae D. Therefore, the involvement of proteinaceous substances in binding process was investigated. Cells of L. mucosae D were digested by three proteolytic enzymes (proteinase K, pronase E, trypsin) and evaluated for time-dependent adhesivity changes to HeLa, Caco-2, and L929 cell lines. Results confirmed that proteins are most likely to play an important role in binding of lactobacilli to eukaryotic cells. One hour after treatment, L. mucosae D was able to overcome the effect of proteolytic cleavage. We assume that it was due to the restoration of its cell-surface binding structures. Co-cultivation of HeLa and L. mucosae D led to protuberance and communication channels formation in eukaryotic cells. [GRAPHICS] .

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Volume of the periodical

149

Issue of the periodical within the volume

5

Country of publishing house

AT - AUSTRIA

Number of pages

7

Pages from-to

893-899

UT code for WoS article

000430848200005

EID of the result in the Scopus database

2-s2.0-85041931844