Stability of total prostate-specific antigen and free prostate-specific antigen after 10 years' storage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F18%3A10376837" target="_blank" >RIV/00216208:11140/18:10376837 - isvavai.cz</a>

Alternative codes found

RIV/00669806:_____/18:10376837

Result on the web

<a href="http://dx.doi.org/10.1177/1724600818789414" target="_blank" >http://dx.doi.org/10.1177/1724600818789414</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/1724600818789414" target="_blank" >10.1177/1724600818789414</a>

Result language

angličtina

Original language name

Stability of total prostate-specific antigen and free prostate-specific antigen after 10 years' storage

Original language description

Introduction: PSA is a serine protease composed of 240 amino acids in a single polypeptide chain and is a routine parameter in prostate cancer diagnostics. The aim of our study was to test the long-term stability of tPSA and fPSA after 10 years&apos; storage at MINUS SIGN 80°C. Materials and methods: We analyzed two aliquots from 55 serum samples. The first was assayed in routine testing at the time of establishing the diagnosis. The second was thawed for further testing after approximately 10 years&apos; storage at MINUS SIGN 80°C. The mean of storage time was 10.41 years (min-max: 9.35-11.40 years). We compared the results of tPSA and fPSA. We calculated the fPSA/tPSA ratio and compared the results of clinical evaluation. Serum tPSA and fPSA levels were assayed using chemiluminescent kits Access Hybritech PSA and free PSA. All measurements were performed using the instrument UniCel(R) DxI 800. Results: tPSA decreased 3.59% on average with a correlation r=0.9213, and fPSA increased at an average of 2.41% with a correlation r=0.9338. The fPSA/tPSA ratio increased 0.80% on average with a correlation r=0.9174. On clinical evaluation, five samples had fallen to a less malignant category and three samples had risen to a higher malignant category compared with the original results. Conclusion: The stability of tPSA and fPSA levels in serum is sufficient after 10 years&apos; storage at MINUS SIGN 80°C. Calculation of the fPSA/tPSA ratio is not recommended due to the change in the category of malignancy of 15% of the samples.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/LM2015089" target="_blank" >LM2015089: Bank of Clinical Specimens</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Biological Markers

ISSN

0393-6155

e-ISSN

—

Volume of the periodical

33

Issue of the periodical within the volume

4

Country of publishing house

IT - ITALY

Number of pages

4

Pages from-to

463-466

UT code for WoS article

000453596300018

EID of the result in the Scopus database

2-s2.0-85060480741