Optimized cutting laser trajectory for laser capture microdissection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F19%3A10394240" target="_blank" >RIV/00216208:11140/19:10394240 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10394240
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VsA3k-lJSw" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VsA3k-lJSw</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11756-019-00234-x" target="_blank" >10.2478/s11756-019-00234-x</a>
Alternative languages
Result language
angličtina
Original language name
Optimized cutting laser trajectory for laser capture microdissection
Original language description
Laser capture microdissection (LCM) is an excellent tool using a laser beam for specific selection and harvesting of cells or cell populations from heterogeneous tissue sections prepared on special slides. The aim of our study was to apply mathematical methods for optimizing the planning of laser trajectory in LCM combining positive and negative selection criteria with adjustable size, weight, importance, and security borders. We developed and tested software tool named CutPlanner to be used in a transparent overlay superimposed to the live camera image independently from the manufacturer of the LCM device. Once optimized, quantified and approved by the user, the resulting outline of the region of interest is directly copied to the laser beam cutting trajectory. The software is made publicly available for non-commercial use to the scientific community and provides a versatile tool for effectively minimizing the length of the laser trajectory to obtain the selected cells without destroying surrounding cells and tissue structures. Saving all the settings allows for performing repeating tasks under similar conditions, especially in uniform and routinely performed LCM applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biologia
ISSN
0006-3088
e-ISSN
—
Volume of the periodical
74
Issue of the periodical within the volume
6
Country of publishing house
SK - SLOVAKIA
Number of pages
8
Pages from-to
717-724
UT code for WoS article
000467648500015
EID of the result in the Scopus database
2-s2.0-85065705281