Frequency of Mutations Associated with Resistance to First- and Second-Line Drugs in Multidrug Resistant Mycobacterium Tuberculosis Isolates
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F20%3A10410202" target="_blank" >RIV/00216208:11140/20:10410202 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/20:43879031
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=26g-E55xnk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=26g-E55xnk</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jgar.2020.03.013" target="_blank" >10.1016/j.jgar.2020.03.013</a>
Alternative languages
Result language
angličtina
Original language name
Frequency of Mutations Associated with Resistance to First- and Second-Line Drugs in Multidrug Resistant Mycobacterium Tuberculosis Isolates
Original language description
INTRODUCTION: Tuberculosis (TB) is considered one of the most fatal diseases worldwide with an estimation of 10.1 million cases (WHO 2018). In this study, Whole genome sequencing (WGS) was used to perform genomic characterization of 40 Mycobacterium tuberculosis (M. tuberculosis) isolates from patients with different nationalities hospitalized in the Czech Republic. MATERIALS AND METHODS: Susceptibility testing for first line drugs was performed. DNA was sequenced using Illumina MiSeq platform. Spoligotypes Single Nucleotide Polymorphisms (SNPs) and mutations in antibiotic resistant genes were detected and phylogenetic analysis was performed. RESULTS: Samples showing phenotypic resistance to at least one drug were 12 to streptomycin, 11 to isoniazid, seven to rifampicin, six to ethambutol and five to pyrazinamide. Phenotypic and genotypic profiles didn't match in all cases suggesting the presence of novel mutation in some cases and low expression of resistant genes in others. The presented phylogeny enables the correct assignation of M. tuberculosis lineages and sub-lineages. Our results suggest that the most dominant lineage in our samples was lineages 4 (33/40). CONCLUSION: To our knowledge, this is the first study using this approach to be done in Czech Republic. Lineage 4 was the predominant lineage identified among our samples. Nevertheless, the dominance of Lineage 4 along with other lineages suggests that infections can be originated from different sources.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Global Antimicrobial Resistance
ISSN
2213-7165
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
September
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
275-282
UT code for WoS article
000572257300037
EID of the result in the Scopus database
2-s2.0-85087002151