New microplate disc volatilization assay for in vitro evaluation of toxicity and antimicrobial activity of volatiles in vapour phase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F25%3A10502966" target="_blank" >RIV/00216208:11140/25:10502966 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=shORGIa7cP" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=shORGIa7cP</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fct.2025.115744" target="_blank" >10.1016/j.fct.2025.115744</a>

Result language

angličtina

Original language name

New microplate disc volatilization assay for in vitro evaluation of toxicity and antimicrobial activity of volatiles in vapour phase

Original language description

In this study, a novel microplate disc volatilization method for in vitro screening of cytotoxic and antibacterial potential of volatile agents in the vapour phase was designed with the aim to assess their therapeutic potential for development of new inhalation therapies. The toxicity and antibacterial activity of plant-derived volatiles (allyl isothiocyanate, 8-quinolinol, thymohydroquinone, thymoquinone) and essential oils (EOs) from Colebrookea oppositifolia leaf and Neolitsea cassia bark were evaluated against human lung fibroblasts MRC-5 and respiratory pathogens. Allyl isothiocyanate and C. oppositifolia EO did not affect the cell viability (IC(50) &gt; 160 μg/cm(3)), on the other hand thymohydroquinone exhibited the highest cytotoxic effect (IC(50) = 5.85 μg/cm(3)) followed by thymoquinone and N. cassia EO (IC(50) = 7.95 and 18.58 μg/cm(3), respectively). 8-Quinolinol possessed the strongest antibacterial effect against Staphylococcus aureus and Heamophilus influenzae with respective minimum inhibitory concentrations of 0.625 and 1.25 μg/cm(3). Calculated therapeutic index (&gt;21.05) suggests relative inhalation safety of this compound. Using a gas chromatography/mass spectrometry analysis, E-cinnmaldehyde, benzaldehyde, and eucalyptol were identified as major components of N. cassia bark oil, while leaf oil of C. oppositifolia consisted predominantly of thunbergene, β-caryophyllene, and m-camphorene. Moreover, chemical profile of N. cassia EO in the vapour phase showed E-cinnamaldehyde, o-cymene, and α-pinene as the most abundant volatiles. The results suggest 8-quinolinol for further research focused on development of inhalation therapies. Additionally, the results demonstrate validity of the microplate disc volatilization method, which allows cost and labour effective high-throughput screening of cytotoxicity and antimicrobial activity of volatiles in vapour phase.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30303 - Infectious Diseases

Project

<a href="/en/project/EF16_019%2F0000787" target="_blank" >EF16_019/0000787: Fighting INfectious Diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food and Chemical Toxicology

ISSN

0278-6915

e-ISSN

1873-6351

Volume of the periodical

206

Issue of the periodical within the volume

December

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

115744

UT code for WoS article

001583006000001

EID of the result in the Scopus database

2-s2.0-105017892525