Method for detection and identification of saccharides by mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F25%3A10504673" target="_blank" >RIV/00216208:11140/25:10504673 - isvavai.cz</a>
Result on the web
<a href="https://patentscope.wipo.int/search/en/WO2025171832" target="_blank" >https://patentscope.wipo.int/search/en/WO2025171832</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Method for detection and identification of saccharides by mass spectrometry
Original language description
The present disclosure provides a method for the detection and/or identification of reducing monosaccharides or oligosaccharides by mass spectrometry in a sample, wherein the reducing saccharide(s) in the sample is (are) reacted with a ligand of general formula I (I), wherein L is selected from the group comprising, wherein n is from 1 to 3,,,, wherein m is 1 or 2, and, wherein in the general formula I the methoxyphenol group is bound to the carbon atom of the linker L, where R1, R2 and R4 are independently selected from the group comprising hydrogen atom and, wherein R3 is selected from the group comprising hydrogen atom and methyl, to form a conjugate by reaction of the aldehyde group(s) of the reducing monosaccharide(s) and oligosaccharide(s) present in the sample with the NH2 group of the ligand, and the conjugate is subsequently subjected to ionization and mass spectrometry measurement. This method can be used also for detection or identification of microbial polysaccharides by mass spectrometry, wherein prior to the reaction with the ligand, the polysaccharides are cleaved enzymatically or by acid hydrolysis to reducing monosaccharides and/or oligosaccharides.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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