Methotrexate released in vitro from bone cement inhibits human stem cell proliferation in S/G2 phase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F10%3A00208540" target="_blank" >RIV/00216208:11150/10:00208540 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/10:00201545

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Methotrexate released in vitro from bone cement inhibits human stem cell proliferation in S/G2 phase

Original language description

Methotrexate (MTX) released from bone cement showed a useful local effect in animal models of bone tumours. However, local toxic reactions such as impaired wound healing were observed in areas surrounding the MTX-loaded implant. Therefore, we hypothesised that MTX released from bone cement would have harmful effects on human mesenchymal stem cells (MSC)-one of the basic components of bone marrow and tissue reparatory processes. Moreover, elution of MTX was calculated from implants prepared either with liquid or powdered MTX. During the 28-day incubation, the cement compounded with liquid MTX showed the highest elution rate of the drug. MTX released from pellets produced a significant decrease in proliferation of MSC as a consequence of a blockade of their cell cycle in the S/G2 phase. These findings indicate impairment of stem cell function in marginal areas surrounding the MTX-loaded cement and may help to explain problems with regeneration of tissues in these locations.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

—

Project

<a href="/en/project/1P05OC061" target="_blank" >1P05OC061: Physiologically based prediction of pharmacokinetics prior to in vivo studies: Focus on hepatic and renal drug elimination</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Orthopaedics

ISSN

0341-2695

e-ISSN

—

Volume of the periodical

34

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

—

UT code for WoS article

000273317100023

EID of the result in the Scopus database

—