Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10104932" target="_blank" >RIV/00216208:11150/11:10104932 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/11:00002471

Result on the web

<a href="http://versita.metapress.com/content/451175j641122359/fulltext.pdf" target="_blank" >http://versita.metapress.com/content/451175j641122359/fulltext.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/v10136-009-0031-7" target="_blank" >10.2478/v10136-009-0031-7</a>

Result language

angličtina

Original language name

Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells

Original language description

In this study we used caffeine, a non-specific ATM inhibitor, and studied its effect on the activation of the proteins involved in cell cycle control and the induction of apoptosis in human T-lymphocyte leukaemic MOLT-4 cells (p53 wt) after irradiation.We evaluated the expression of the tumour-suppressor p53, the cell cycle regulator p21, and the anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1). After treatment with 2 mM caffeine, the cells were irradiated by 1 or 3 Gy. The ATM/p53 pathway was suppressed, which led to increased apoptosis accompanied by a p53-independent decrease in Mcl-1. It also caused down-regulation of p21, which possibly contributed to the shortened cell cycle arrest necessary for effective DNA repair and thus impeded radio-resistance. Caffeine promotes the cytotoxic effect of ionising radiation and provides a possible platform for the development of new anti-cancer therapeutics known as radio-sensitizers.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomedicine

ISSN

1214-021X

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

49-56

UT code for WoS article

000284780800004

EID of the result in the Scopus database

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