Role of Transplanted Bone Marrow Cells in Response to Skeletal Muscle Injury

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10104934" target="_blank" >RIV/00216208:11150/11:10104934 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/11:00002607
Result on the web
<a href="http://fb.cuni.cz/file/5610/FB2011A0033.pdf" target="_blank" >http://fb.cuni.cz/file/5610/FB2011A0033.pdf</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Role of Transplanted Bone Marrow Cells in Response to Skeletal Muscle Injury
Original language description
In this work, recipient mice were intramuscularly injected with cardiotoxin, then whole-body lethally irradiated to eradicate satellite cells in their injured tibialis anterior muscles and to suppress hematopoiesis and subsequently, intravenously transplanted with mouse donor lacZ+ bone marrow cells with the aim to investigate role of exogenous bone marrow cells in response to skeletal muscle injury. Seven to 33 days after grafting, recipient tibialis anterior muscles were examined to detect donor-derived X-gal+ cells and analyzed by quantitative PCR. In injured recipients'' muscles, X-gal positivity was identified 14 and 33 days after grafting in some infiltrating neutrophils and macrophages, infrequently in fibroblasts of endomysium and in many largemultinucleated cells (devoid of myogenic markers desmin and nestin) resembling foreign body giant cells situated in vicinity to necrotic muscle fibers. qPCR confirmed the presence of lacZ+ cells in injured recipients'' muscles. Our resul
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
—

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)