The role of p38 in irinotecan-induced DNA damage and apoptosis of colon cancer cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10133987" target="_blank" >RIV/00216208:11150/13:10133987 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0027510713000158" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0027510713000158</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mrfmmm.2013.02.002" target="_blank" >10.1016/j.mrfmmm.2013.02.002</a>
Alternative languages
Result language
angličtina
Original language name
The role of p38 in irinotecan-induced DNA damage and apoptosis of colon cancer cells
Original language description
The role of p38 in irinotecan (CPT-11)-induced damage and cell death in colon cancer cell line SW620 was investigated. We demonstrate that CPT-11 treatment activates p38 in exposed cells, however with concentration dependent dynamics and differing consequences. Higher CPT-11 concentrations induce a massive early but relatively short-lasting p38 activity leading to apoptosis mediated by mitochondria and caspases. Pharmacological or siRNA inhibition of p38 then significantly prevents CPT-11-dependent celldeath. Conversely, lower CPT-11 concentrations activate p38 in a delayed, however sustained manner, with apoptosis occurring only in a fraction of cells and in the absence of significant autophagy. Blocking p38 in thus treated cells increases their sensitivity toward CPT-11 and increases cell death. In summary, our results confirm the involvement of p38 in colon cancer cells response to CPT-11 while indicating a varying role of p38 in the final biological response.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mutation Research
ISSN
0027-5107
e-ISSN
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Volume of the periodical
741-742
Issue of the periodical within the volume
January - February
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
27-34
UT code for WoS article
000318456400004
EID of the result in the Scopus database
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