Inhibition of ATR kinase with the selective inhibitor VE-821 results in radiosensitization of cells of promyelocytic leukaemia (HL-60)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10188911" target="_blank" >RIV/00216208:11150/13:10188911 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/13:00399421 RIV/60162694:G44__/13:43874889

Result on the web

<a href="http://dx.doi.org/10.1007/s00411-013-0486-5" target="_blank" >http://dx.doi.org/10.1007/s00411-013-0486-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00411-013-0486-5" target="_blank" >10.1007/s00411-013-0486-5</a>

Result language

angličtina

Original language name

Inhibition of ATR kinase with the selective inhibitor VE-821 results in radiosensitization of cells of promyelocytic leukaemia (HL-60)

Original language description

We compared the effects of inhibitors of kinases ATM (KU55933) and ATR (VE-821) (incubated for 30 min before irradiation) on the radiosensitization of human promyelocyte leukaemia cells (HL-60), lacking functional protein p53. VE-821 reduces phosphorylation of check-point kinase 1 at serine 345, and KU55933 reduces phosphorylation of check-point kinase 2 on threonine 68 as assayed 4 h after irradiation by the dose of 6 Gy. Within 24 h after gamma-irradiation with a dose of 3 Gy, the cells accumulated inthe G2 phase (67 %) and the number of cells in S phase decreased. KU55933 (10 ?M)) did not affect the accumulation of cells in G2 phase and did not affect the decrease in the number of cells in S phase after irradiation. VE-821 (2 and 10 ?M)) reduced the number of irradiated cells in the G2 phase to the level of non-irradiated cells and increased the number of irradiated cells in S phase, compared to irradiated cells not treated with inhibitors. In the 144 h interval after irradiation w

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Radiation and Environmental Biophysics

ISSN

0301-634X

e-ISSN

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Volume of the periodical

52

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

471-479

UT code for WoS article

000326074900005

EID of the result in the Scopus database

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