Changes in the response of MCF-7 cells to ionizing radiation after the combination of ATM and DNA-PK inhibition

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F15%3A10293814" target="_blank" >RIV/00216208:11150/15:10293814 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/15:43875359 RIV/00216275:25310/15:39900079

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs12032-015-0591-1" target="_blank" >http://link.springer.com/article/10.1007%2Fs12032-015-0591-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12032-015-0591-1" target="_blank" >10.1007/s12032-015-0591-1</a>

Result language

angličtina

Original language name

Changes in the response of MCF-7 cells to ionizing radiation after the combination of ATM and DNA-PK inhibition

Original language description

The aim of the present study is to evaluate the role of ATM (KU55933) and DNA-PK (NU7441) inhibitors in the repair of double-strand breaks and downstream signaling of DNA damage introduced by ionizing radiation. The irradiation of MCF-7 cells alone increased the proportion of cells in the G1 phase in comparison with mock-treated cells. After ATM inhibitor pretreatment, the cells were more accumulated in the G2 phase, whereas DNA-PK inhibitor application increased the percentage of cells in the G1 phase.ATM and DNA-PK inhibitor application alone increased the sensitivity of MCF-7 cells to ionizing radiation; however, combining both inhibitors together resulted in a further enhancement of cell death. Unexpectedly, combining both inhibitors decreased thepercentage of senescent cells and increased G2 cell cycle arrest 3 days after treatment. After irradiation, the p21 protein was increased and Chk1 and Chk2 were activated. These proteins were not increased in cells pretreated with the AT

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Oncology

ISSN

1357-0560

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

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UT code for WoS article

000352152000002

EID of the result in the Scopus database

2-s2.0-84925590335