Detection of Mycoplasma Contamination Directly from Culture Supernatant Using Polymerase Chain Reaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F16%3A10330483" target="_blank" >RIV/00216208:11150/16:10330483 - isvavai.cz</a>
Result on the web
<a href="http://fb.cuni.cz/file/5821/fb2016a0025.pdf" target="_blank" >http://fb.cuni.cz/file/5821/fb2016a0025.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Detection of Mycoplasma Contamination Directly from Culture Supernatant Using Polymerase Chain Reaction
Original language description
Ensuring mycoplasma-free cell culture is of prime importance as they severely affect cellular characteristics leading to experimental artefacts and spurious results. Various methods persist for mycoplasma detection; out of the whole array of methods plymerase chain reaction (PCR) is the most favoured one because it is highly sensitive, specific and quick. The PCR-based detection procedure involves three steps: cell culture supernatant collection, DNA isolation, and PCR. We have modified this procedure so that cell culture supernatant can directly be used for PCR without the need for DNA extraction. This modification makes the procedure quicker and more sensitive because loss of mycoplasma DNA is prevented and this loss becomes more significant when the level of mycoplasma contamination is very low.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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