High soluble endoglin levels do not induce changes in structural parameters of mouse heart

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F17%3A10365231" target="_blank" >RIV/00216208:11150/17:10365231 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/17:10365231

Result on the web

<a href="http://link.springer.com/article/10.1007/s00380-017-0976-5" target="_blank" >http://link.springer.com/article/10.1007/s00380-017-0976-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00380-017-0976-5" target="_blank" >10.1007/s00380-017-0976-5</a>

Result language

angličtina

Original language name

High soluble endoglin levels do not induce changes in structural parameters of mouse heart

Original language description

A soluble form of endoglin (sEng) released into the circulation was suggested to be a direct inducer of endothelial dysfunction, inflammation and contributed to the development of hypertension by interfering with TGF-beta signaling in cardiovascular pathologies. In the present study, we assessed the hypothesis that high sEng level-induced hypertension via a possible sEng interference with TGF-beta signaling pathways may result in inflammatory, structural or fibrotic changes in hearts of Sol-Eng+ mice (mice with high levels of soluble endoglin) fed either chow or high-fat diet. Female Sol-Eng+ mice and their age matched littermates with low plasma levels of sEng were fed either chow or high-fat diet (HFD). Heart samples were subsequently analyzed by histology, qRT-PCR and Western blot analysis. In this study, no differences in myocardial morphology/hypertrophy and possible fibrotic changes between Sol-Eng+ mice and control mice were detected on both chow and HFD. The presence of sEng did not significantly affect the expression of selected members of TGF-beta signaling (membrane endoglin, TGF beta RII, ALK-5, ALK-1, Id-1, PAI-1 and activated Smad proteins-pSmad 1,5 and pSmad 2,3), inflammation, heart remodeling (PDGFb, Col1A1) and endothelial dysfunction (VCAM-1, ICAM-1) in the hearts of Sol-Eng+ mice compared to control mice on both chow and high-fat diet. High levels of soluble endoglin did not affect microscopic structure (profibrotic and degenerative cardiomyocyte changes), and specific parts of TGF-beta signaling, endothelial function and inflammation in the heart of Sol-Eng+ mice fed both chow diet or HFD. However, we cannot rule out a possibility that a long-term chronic exposure (9 months and more) to soluble endoglin alone or combined with other cardiovascular risk factors may contribute to alterations of heart function and structure in Sol-Eng+ mice, which is the topic in our lab in ongoing experiments.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/GA15-24015S" target="_blank" >GA15-24015S: Tissue and soluble endoglin and their importance in endothelial dysfunction and atherogenesis in vivo and in vitro</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Heart and Vessels

ISSN

0910-8327

e-ISSN

—

Volume of the periodical

32

Issue of the periodical within the volume

8

Country of publishing house

JP - JAPAN

Number of pages

12

Pages from-to

1013-1024

UT code for WoS article

000406175700014

EID of the result in the Scopus database

2-s2.0-85017169179