Characteristics of Human Natal Stem Cells Cultured in Allogeneic Medium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F18%3A10383573" target="_blank" >RIV/00216208:11150/18:10383573 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/18:10383573

Result on the web

<a href="https://doi.org/10.1590/0103-6440201802388" target="_blank" >https://doi.org/10.1590/0103-6440201802388</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1590/0103-6440201802388" target="_blank" >10.1590/0103-6440201802388</a>

Result language

angličtina

Original language name

Characteristics of Human Natal Stem Cells Cultured in Allogeneic Medium

Original language description

Recently, human natal dental pulp stem cells (hNDP-SCs) have been characterized in vitro and it has been shown that they satisfy criteria defining human mesenchymal stromal cells (MSCs), as proposed by the International Society for Cellular Therapy. However, these results were reached in the presence of xenogeneic expansion medium, which has the potential to alter the cells&apos; functional capacity. To determine the validity of the previously reported hNDP-SCs characteristics for human cell therapy, we have cultured hNDP-SCs in allogeneic expansion medium. Two hNDP-SC lineages were isolated from vital natal teeth, donated by a healthy newborn female and cultured in 2% platelet rich plasma (PRP). Analysis of the phenotypic expressions, proliferation rates, viability, telomerase length and in vitro adipogenic, osteogenic and chondrogenic differentiation potentials of two hNDP-SCs lineages (Zn001 and Zn002) were performed. Both lineages displayed similar morphology, proliferation rates, adipogenic, chondrogenic and osteogenic differentiation potential. Telomere shortening by 41.0% and 13.49% occurred from 3rd till 14th passage for lineages Zn001 and Zn002 respectively. Viability of both lineages was higher than 90%. Flow cytometry demonstrated that both lineages were positive to the majority of tested markers, including markers, which were negatively, expressed when hNDP-SCs were cultured previously in xenogeneic medium. Using immune-cytochemistry the cells were shown to express beta III-tubulin, nestin, neurofilaments and Nanog. PRP used as allogeneic medium is suitable for cultivation of hNDP-SCs.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Brazilian Dental Journal

ISSN

0103-6440

e-ISSN

—

Volume of the periodical

29

Issue of the periodical within the volume

5

Country of publishing house

BR - BRAZIL

Number of pages

8

Pages from-to

427-434

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85059814782