Variation of selected genotoxic and epigenetic markers due to therapeutic exposure to PAHs and ultraviolet radiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F20%3A10418681" target="_blank" >RIV/00216208:11150/20:10418681 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/20:10418681 RIV/00179906:_____/20:10418681
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=7l9R_MlJCS" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=7l9R_MlJCS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/BLL_2020_093" target="_blank" >10.4149/BLL_2020_093</a>
Alternative languages
Result language
angličtina
Original language name
Variation of selected genotoxic and epigenetic markers due to therapeutic exposure to PAHs and ultraviolet radiation
Original language description
BACKGROUND: Goeckerman therapy (GT) of psoriasis involves dermal application of crude coal tar containing polycyclic aromatic hydrocarbons (PAHs) and exposure to ultraviolet radiation (UVR). Little is known about GT influence on DNA epigenetics. OBJECTIVE: The study aim was to discover epigenetic mechanisms altered by the exposure related to the GT of psoriasis. METHODS: Observed group of patients with plaque psoriasis (n = 23) was treated by GT with 3 % CCT. Before and after GT, we analyzed the levels of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts (BPDE-DNA), p53 protein in serum, 5-methylcytosine (5-mC, global DNA methylation), and methylation in selected CpG sites of p53 gene. RESULTS: We found a significant increase in the levels of BPDE-DNA (p < 0.01) and serum levels of p53 protein (p < 0.01) after GT, and an insignificant decrease in the percentage of 5-mC in peripheral blood DNA. Methylation of p53 CpG sites was affected neither by psoriasis nor by GT. The study confirmed good effectiveness of GT (significantly reduced psoriasis area and severity index; p < 0.001). CONCLUSION: Our findings indicate that there is a significantly increased genotoxic hazard related to the exposure of PAHs and UV radiation after GT of psoriasis. However, global DNA methylation and p53 gene methylation evade the effect of GT, as they remained unchanged (Tab. 4, Fig. 3, Ref. 50). Text in PDF www.elis.sk
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30216 - Dermatology and venereal diseases
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bratislavské lekárske listy / Bratislava Medical Journal
ISSN
0006-9248
e-ISSN
—
Volume of the periodical
121
Issue of the periodical within the volume
8
Country of publishing house
SK - SLOVAKIA
Number of pages
7
Pages from-to
558-564
UT code for WoS article
000571574800007
EID of the result in the Scopus database
2-s2.0-85088840227