Simple automated generation of gradient elution conditions in sequential injection chromatography using monolithic column

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F11%3A10100196" target="_blank" >RIV/00216208:11160/11:10100196 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0039914011000622" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0039914011000622</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2011.01.029" target="_blank" >10.1016/j.talanta.2011.01.029</a>

Result language
angličtina
Original language name
Simple automated generation of gradient elution conditions in sequential injection chromatography using monolithic column
Original language description
The paper deals with the concept of simple automated creation of gradient profile of the mobile phase for gradient-elution sequential injection chromatography (GE-SIC). The feasibility and merits of this concept are demonstrated on the separation and simultaneous assay of indomethacin as active principle and of its two degradation products (5-methoxy-2-methylindoleacetic acid and 4-chloro-benzoic acid) in a topical pharmaceutical formulation. The GE-SIC separation was performed with a FIAlab (R) 3000 SIC set-up (USA) equipped with an Onyx (TM) Monolithic C18 (25 mm x 4.6 mm, Phenomenex (R)) column, a six-port selection valve, a 5-mL syringe pump and a fiber-optics UV CCD detector. Ketoprofen was used as an internal standard (IS). The gradient elution was achieved by automated reproducible mixing of acetonitrile and aqueous 0.2% phosphoric acid in the holding coil of the SIC system.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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