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Import and efflux of flubendazole in Haemonchus contortus strains susceptible and resistant to anthelmintics

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124809" target="_blank" >RIV/00216208:11160/12:10124809 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S0304401712000805" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0304401712000805</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.vetpar.2012.02.008" target="_blank" >10.1016/j.vetpar.2012.02.008</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Import and efflux of flubendazole in Haemonchus contortus strains susceptible and resistant to anthelmintics

  • Original language description

    Drug entry into the body of a helminth is a key factor in the efficacy of anthelmintics. The present project was designed to study the ex vivo uptake and efflux of the benzimidazole anthelmintic flubendazole (FLU) in four strains of H. contortus: the ISEstrain (fully susceptible to anthelmintics), the ISE-S strain (resistant to ivermectin), the BR strain (resistant to benzimidazoles) and the WR strain (multi-resistant). The transport of FLU between dead and living nematodes was also compared as well asthe effect of verapamil, an inhibitor of the main efflux ABCB1 transporter (P-glycoprotein), on FLU accumulation in nematodes. The obtained results showed that FLU is able to effectively enter H. contortus adults due to high FLU lipophilicity. Passive diffusion is probably the only mechanism in both FLU import and efflux from nematodes. No differences in FLU transport were found among four H. contortus strains with different sensitivity to anthelmintics. No active FLU efflux from H. con

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP502%2F10%2F0217" target="_blank" >GAP502/10/0217: Drug-resistance development in helminths - possible mechanisms and defense</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Veterinary Parasitology

  • ISSN

    0304-4017

  • e-ISSN

  • Volume of the periodical

    187

  • Issue of the periodical within the volume

    3-4

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    7

  • Pages from-to

    473-479

  • UT code for WoS article

    000307323700017

  • EID of the result in the Scopus database