Determination of phenolic acids in plant extracts using CZE with on-line transient isotachophoretic preconcentration

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10145913" target="_blank" >RIV/00216208:11160/13:10145913 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201200806/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201200806/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201200806" target="_blank" >10.1002/jssc.201200806</a>

Result language
angličtina
Original language name
Determination of phenolic acids in plant extracts using CZE with on-line transient isotachophoretic preconcentration
Original language description
A novel transient ITP-CZE for preconcentration and determination of seven phenolic acids (caffeic acid, cinnamic acid, p-coumaric acid, ferulic acid, protocatechuic acid, syringic acid, and vanilic acid) was developed and validated. Effects of several factors such as control of EOF, pH and buffer concentration, addition of organic solvents and CDs, and conditions for sample injection were investigated. Sample self-stacking was applied by means of induction of transient ITP, which was realized by addingsodium chloride into the sample. The CZE was realized in 200 mM borate buffer ((s)(w)pH 9.2) containing 37.5% methanol, 0.001% hexadimethrine bromide, and 15 mM 2-hydroxypropyl-beta-CD. Under the optimal conditions for analysis, analytes were separated within 20 min. Linearity was tested for each compound in the concentration range of 0.1-10 mu g/mL (R = 0.9906-0.9968) and the detection limits (S/N = 3) ranged from 11 ng/mL (protocatechuic acid) to 31 mu g/mL (syringic acid). The validat
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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