Modulatory Effects of Quercetin and Rutin on the Activity, Expression and Inducibility of CYP1A1 in Intestinal HCT-8 Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10189501" target="_blank" >RIV/00216208:11160/13:10189501 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/ptr.4992/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/ptr.4992/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ptr.4992" target="_blank" >10.1002/ptr.4992</a>

Result language
angličtina
Original language name
Modulatory Effects of Quercetin and Rutin on the Activity, Expression and Inducibility of CYP1A1 in Intestinal HCT-8 Cells
Original language description
Flavonoids, plant secondary metabolites present in fruits and vegetables, show antioxidant and anti-tumorigenic effects in vitro, but their poor absorption from gastrointestinal tract limits their systemic efficacy in humans. On the other hand, flavonoids could protect intestinal cells against carcinogens by their potential to inhibit the enzymes metabolizing pre-carcinogenic compounds (e.g. CYP1A) to reactive ones. This work was designed to test the effect of quercetin (the most abundant flavonoid) andrutin (the most abundant glycosidic form) on the activity, expression and inducibility of CYP1A in intestinal HCT-8 cells. CYP1A enzymatic activity was measured by ethoxyresorufin-O-deethylase (EROD) activity, CYP1A protein expression was detected by western blotting. The effect of flavonoids on viability of cells was examined by neutral red uptake test. No cytotoxic effect of flavonoids up to 50M concentration was observed. Quercetin significantly inhibited EROD activity in the cells,
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
<a href="/en/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centre of drug-dietary supplements interactions and nutrigenetics</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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