Purification and reconstitution of human membrane-bound DHRS7 (SDR34C1) from Sf9 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10281983" target="_blank" >RIV/00216208:11160/14:10281983 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S104659281300257X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S104659281300257X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pep.2013.11.013" target="_blank" >10.1016/j.pep.2013.11.013</a>
Alternative languages
Result language
angličtina
Original language name
Purification and reconstitution of human membrane-bound DHRS7 (SDR34C1) from Sf9 cells
Original language description
Dehydrogenase/reductase SDR family member 7 (DHRS7, SDR34C1, retSDR4) is one of the many endoplasmic reticulum bound members of the SDR superfamily. Preliminary results indicate its potential significance in human metabolism. DHRS7 containing TEV-cleavable His(10) and FLAG-tag expressed in the Sf9 cell line was solubilised, purified, and reconstituted into liposomes to enable the improved characterisation of this enzyme in the future. Igepal CA-630 was determined to be the best detergent for the solubilisation process. The solubilised DHRS7 was purified using affinity chromatography, and the purified enzyme was subjected to TEV cleavage of the affinity tags and then repurified using subtractive Ni-IMAC. The cleaved and uncleaved versions of DHRS7 weresuccessfully reconstituted into liposomes. In addition, using tobacco specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as the substrate, the cleaved liposomal DHRS7 was found to be inactive, whereas the pure and un
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Establishment of Research Team Focused on Experimental and Applied Biopharmacy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Expression and Purification
ISSN
1046-5928
e-ISSN
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Volume of the periodical
95
Issue of the periodical within the volume
March
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
44-49
UT code for WoS article
000332192900007
EID of the result in the Scopus database
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