Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364892" target="_blank" >RIV/00216208:11160/17:10364892 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22310/17:43914226 RIV/60461373:22810/17:43914226
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S030146221630463X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S030146221630463X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2017.03.004" target="_blank" >10.1016/j.bpc.2017.03.004</a>
Alternative languages
Result language
angličtina
Original language name
Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains
Original language description
The Stratum corneum (SC) prevents water loss from the body and absorption of chemicals. SC intercellular spaces contain ceramides (Cer), free fatty acids (FFA), cholesterol (Chol) and cholesteryl sulfate (CholS). Cer with "very long" acyl chains (for example, N-lignoceroyl-sphingosine, CerNS24) are important for skin barrier function, whereas increased levels of "long" acyl Cer (for example, N-palmitoyl-sphingosine, CerNS16) occur in patients suffering from atopic eczema or psoriasis. We studied the impact of the replacement of CerNS24 by CerNS16 on the barrier properties and microstructure of model SC lipid membranes composed of Cer/FFA/Chol/CholS. Membranes containing the long CerNS16 were significantly more permeable to water (by 38-53%), theophylline (by 50-55%) and indomethacin (by 83-120%) than those containing the very long CerNS24 (either with lignoceric acid or a mixture of long to very long chain FFA). Langmuir monolayers with CerNS24 were more condensed than with CerNS16 and atomic force microscopy showed differences in domain formation. X-ray powder diffraction revealed that CerNS24-based membranes formed one lamellar phase and separated Chol, whereas the CerNS16-based membranes formed up to three phases and Chol. These results suggest that replacement of CerNS24 by CerNS16 has a direct negative impact on membrane structure and permeability.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Chemistry
ISSN
0301-4622
e-ISSN
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Volume of the periodical
224
Issue of the periodical within the volume
May
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
20-31
UT code for WoS article
000401597100002
EID of the result in the Scopus database
2-s2.0-85016137333