Determination of fluoroquinolones in fishes using microwave-assisted extraction combined with ultra-high performance liquid chromatography and fluorescence detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365270" target="_blank" >RIV/00216208:11160/17:10365270 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0889157516302289" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0889157516302289</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2016.12.012" target="_blank" >10.1016/j.jfca.2016.12.012</a>
Alternative languages
Result language
angličtina
Original language name
Determination of fluoroquinolones in fishes using microwave-assisted extraction combined with ultra-high performance liquid chromatography and fluorescence detection
Original language description
A new analytical methodology based on ultra-high performance liquid chromatography with fluorescence detection (UHPLC-FD) has been developed for the determination of five fluoroquinolone antibiotics (FQs) used in aquaculture (norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin and sarafloxacin) in fish muscles (e.g. gilthead seabream (Sparus aurata)). The extraction and determination process was carried out by combining microwave-assisted extraction (MAE) along with a clean-up and pre-concentration step with solid phase extraction (SPE) followed by UHPLC-FD detection. The effects of different variables on MAE-SPE were studied and optimized using an experimental design. Validation was made in accordance with Decision 2002/657/EC. CC alpha and CC beta were determined according to the maximum residue limit (MRL). The method detection limit (MDL) of the entire process ranged between 0.1 and 6.0 ng g(-1). The recoveries obtained at two spiked concentrations levels (25 and 250 ng g(-1)) were greater than 90%, and the relative standard deviation was less than 8.7%. The developed methodology was successfully applied for the evaluation of the presence of FQs in fish muscle samples from aquaculture farms bought in various commercial shops in Gran Canaria Island (Spain).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
<a href="/en/project/EE2.3.30.0061" target="_blank" >EE2.3.30.0061: Increasing of the R&D capacity at Charles University through new positions for graduates of doctoral studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food Composition and Analysis
ISSN
0889-1575
e-ISSN
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Volume of the periodical
56
Issue of the periodical within the volume
March
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
140-146
UT code for WoS article
000393526600018
EID of the result in the Scopus database
2-s2.0-85006380190