Automated Sequential Injection Method for Determination of Caffeine in Coffee Drinks
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10382499" target="_blank" >RIV/00216208:11160/18:10382499 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s12161-017-0982-3" target="_blank" >http://link.springer.com/article/10.1007/s12161-017-0982-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12161-017-0982-3" target="_blank" >10.1007/s12161-017-0982-3</a>
Alternative languages
Result language
angličtina
Original language name
Automated Sequential Injection Method for Determination of Caffeine in Coffee Drinks
Original language description
An automated sequential injection analysis spectrophotometric assay for the determination of purine alkaloids in coffee drinks was developed. The sample was treated with a carrez reagent for matrix suppression followed by filtration; subsequently, alkaloids were separated from organic acids using a short C18 monolithic column (10 x 4.6 mm). The flow rate of the separation step was 10 mu L s(-1) with 10% v/v of methanol as the mobile phase. The sum of alkaloids evaluated as caffeine was detected at 274 nm. The influence of the main parameters affecting the quantification of purine alkaloids was optimized. One sample analysis lasted 15 min when aspirated in triplicate. The linear range was 1-15 mg L-1, and the determination coefficient (r (2)) was 0.9969. The limit of detection and limit of quantitation were 0.128 and 0.425 mg L-1, respectively. The repeatability evaluated as the relative standard deviation (RSD) was 3.58% (n = 12, 10 mg L-1). Under optimal conditions, the method was successfully applied to determine purine alkaloids in different real samples including soluble coffee, coffee from an espresso machine, and brewed coffee drinks.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF15_003%2F0000465" target="_blank" >EF15_003/0000465: Establishment of Specialized Team for Advanced Research on Separation Science</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food Analytical Methods
ISSN
1936-9751
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
111-118
UT code for WoS article
000418836200012
EID of the result in the Scopus database
2-s2.0-85021801331