Investigating chirality in quorum sensing by analysis of Burkholderia cepacia and Vibrio fischeri with comprehensive chiral LC-MS/MS and GC-MS/MS methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F23%3A10471937" target="_blank" >RIV/00216208:11160/23:10471937 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=-fRzIj9vT" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=-fRzIj9vT</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsle/fnad011" target="_blank" >10.1093/femsle/fnad011</a>

Result language

angličtina

Original language name

Investigating chirality in quorum sensing by analysis of Burkholderia cepacia and Vibrio fischeri with comprehensive chiral LC-MS/MS and GC-MS/MS methods

Original language description

N-acyl homoserine lactones (N-HLs) are signaling molecules used by Gram-negative bacteria in a phenomenon called quorum sensing. Bacteria will detect N-HLs as a way of monitoring their population which, upon reaching a critical level, will express a specific phenotype. An example is the expression of bioluminescence by Vibrio fischeri. Most studies have not considered the chirality of these molecules nor have they used highly sensitive detection methods. Here, the production of d,l-N-HLs are monitored for V. fischeri, Burkholderia cepacia, Pseudomonas fluorescens, and P. putida, using highly sensitive tandem mass spectrometry analysis. Novel N-HLs are reported for both V. fischeri and B. cepacia, including a plethora of previously unknown d-N-HLs, including the first d-N-HLs containing oxo and hydroxy functionalities. Anomalously, N-HLs were not detected in any cultures of P. fluorescens and P. putida, which are species that previously were reported to produce N-HLs. However, it is apparent that differences in the reported occurrence and levels of N-HLs can result from (a) different strains of bacteria, (b) different growth media and environmental conditions, and (c) sometimes false-positive results from detection methodologies. Time studies of V. fischeri suggest the possibility that separate synthetic and elimination pathways exist between d- and l-N-HLs. Possible biological processes that could be the source of d-N-HL production are considered. New &quot;mirror image&quot; bacterial signaling molecules have been discovered and their possible biological roles discussed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

1574-6968

Volume of the periodical

370

Issue of the periodical within the volume

January

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

fnad011

UT code for WoS article

000945682700005

EID of the result in the Scopus database

2-s2.0-85150000179