Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A10000949" target="_blank" >RIV/00216208:11310/09:10000949 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/09:00328462

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants

Original language description

The effect of viral infection on the regulation of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in Nicotiana tabacum L. leaves was studied. PEPC activity was 3 times higher in infected plant leaves compared to healthy plants. The reason for the increase of PEPC activity as a consequence of PVYNTN infection was studied. The amount of PEPC protein did not differ in control and PVYNTN-infected plants. Changes in post-translational modification of PEPC by phosphorylation were evaluated by comparing activity of the native and the dephosphorylated enzyme. The infected plants were characterized by a higher decrease of the enzyme activity after its dephosphorylation, which indicated a higher phosphorylation level. Immunochemical detection of phosphoproteins showed a more intensive band corresponding to PEPC from the infected material. This strengthens the hypothesis of an infection-related phosphorylation, which could be part of the plant's response to pathogen attack.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biological Chemistry

ISSN

1431-6730

e-ISSN

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Volume of the periodical

390

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

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UT code for WoS article

000264921500005

EID of the result in the Scopus database

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