Cooperation between Subunits Is Essential for High-Affinity Binding of N-Acetyl-D-hexosamines to Dimeric Soluble and Dimeric Cellular Forms of Human CD69

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F10%3A10057594" target="_blank" >RIV/00216208:11310/10:10057594 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/10:00350684

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Cooperation between Subunits Is Essential for High-Affinity Binding of N-Acetyl-D-hexosamines to Dimeric Soluble and Dimeric Cellular Forms of Human CD69

Original language description

CD69 is an earliest lymphocyte activation antigen and a universal leukocyte triggering molecule expressed at sites of active immune response. The binding of GlcNAc to the dimeric human CD69 was followed by equilibrium dialysis, fluorescence titration, and NMR. Clear cooperation was observed in the high-affinity binding (Kd=4.0 X 10-7 M) of the carbohydrate to two subunits of the dimeric CD69 (Hill coefficient 1.94). A control monosaccharide ManNAc was not bound by human CD69, and both monosaccharides had no effects on the structure of the receptor. However, a monomeric CD69 obtained by mutating Q93 and R134 at the dimer interface exhibited a much lower affinity for GlcNAc (Kd=1.3 X 10-5 M) and no cooperativity (Hill coefficient 1.07).

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemistry

ISSN

0006-2960

e-ISSN

—

Volume of the periodical

49

Issue of the periodical within the volume

19

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

—

UT code for WoS article

000277398100008

EID of the result in the Scopus database

—