Three metallothionein isoforms and sequestration of intracellular silver in the hyperaccumulator Amanita strobiliformis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10082569" target="_blank" >RIV/00216208:11310/11:10082569 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/11:00360138 RIV/61389005:_____/11:00360138 RIV/60461373:22330/11:43891712
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2010.03634.x/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2010.03634.x/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1469-8137.2010.03634.x" target="_blank" >10.1111/j.1469-8137.2010.03634.x</a>

Result language
angličtina
Original language name
Three metallothionein isoforms and sequestration of intracellular silver in the hyperaccumulator Amanita strobiliformis
Original language description
Metallothioneins (MTs) are cysteine-rich peptides involved in heavy metal tolerance of many eukaryotes. Here, we examined their involvement in intracellular binding of silver (Ag) in the ectomycorrhizal fungus Amanita strobiliformis. The Ag complexes andtheir peptide ligands were characterized using chromatography and mass spectrometry. The full-length coding sequences obtained from a cDNA library were used for complementation assays in yeast mutant strains. Abundance of respective transcripts in A. strobiliformis was measured by quantitative real-time reverse-transcribed polymerase chain reaction (qRT-PCR). Ag-speciation analyses showed that intracellular Ag was in wild-grown fruit bodies and cultured extraradical mycelia of A. strobiliformis sequestered by metallothioneins. The determined sequence of the peptide facilitated isolation of three cDNA clones, AsMT1a, AsMT1b and AsMT1c. These encode isomorphic MTs consisting of 34 amino acid residues and sharing 82% identity.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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