Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10100566" target="_blank" >RIV/00216208:11310/11:10100566 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/11:00370402
Result on the web
<a href="http://dx.doi.org/10.1155/2011/634253" target="_blank" >http://dx.doi.org/10.1155/2011/634253</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2011/634253" target="_blank" >10.1155/2011/634253</a>
Alternative languages
Result language
angličtina
Original language name
Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis
Original language description
The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHC? was represented by the lower band with higher mobility and MyHC? by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHC? possessed a higher mobility rate than the MyHC? isoform. Our data highlighted the importance of the careful identification of the MyHC? and MyHC? isoforms analyzed by the SDS-PAGE.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biomedicine and Biotechnology
ISSN
1110-7243
e-ISSN
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Volume of the periodical
2011
Issue of the periodical within the volume
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Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1-9
UT code for WoS article
000298749900001
EID of the result in the Scopus database
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