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Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10100566" target="_blank" >RIV/00216208:11310/11:10100566 - isvavai.cz</a>

  • Alternative codes found

    RIV/67985823:_____/11:00370402

  • Result on the web

    <a href="http://dx.doi.org/10.1155/2011/634253" target="_blank" >http://dx.doi.org/10.1155/2011/634253</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1155/2011/634253" target="_blank" >10.1155/2011/634253</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Electrophoretic Mobility of CardiacMyosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis

  • Original language description

    The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHC? was represented by the lower band with higher mobility and MyHC? by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHC? possessed a higher mobility rate than the MyHC? isoform. Our data highlighted the importance of the careful identification of the MyHC? and MyHC? isoforms analyzed by the SDS-PAGE.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    ED - Physiology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biomedicine and Biotechnology

  • ISSN

    1110-7243

  • e-ISSN

  • Volume of the periodical

    2011

  • Issue of the periodical within the volume

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    1-9

  • UT code for WoS article

    000298749900001

  • EID of the result in the Scopus database